DNA sequencing is the technology that is used in modern molecular research to determine the exact order or sequence of nucleotides in a DNA molecule. This process has brought a revolution in biology, genetics, medicine, and many other fields. Also. it has enabled scientists to analyze and comprehend genetic information in a more innovative way.

In most cases, DNA sequencing involves breaking the DNA into small pieces, studying the sequence of each piece, and then assembling the pieces back together to rebuild the original sequence. Today, researchers majorly use two methods for DNA sequencing. One is Sanger sequencing and the other one is Next-generation sequencing or NGS. The widely used next-generation sequencing uses advanced techniques to rapidly sequence large amounts of DNA.

Use of DNA Sequencing :

DNA sequencing services are associated with various applications, including identifying genetic variations related to diseases, understanding the evolution and diversity of species, and developing new and more advanced treatments and drugs for genetic disorders.

Types of DNA Sequencing :

Sanger sequencing is a DNA sequencing method developed in 1977. It basically involves chain termination and DNA synthesis that help to read the sequence of a DNA molecule. Amplifying the DNA sequence of interest, naming it, and then separating the fragments by size with the help of gel electrophoresis are the steps of Sanger sequencing.

Next-generation Sequencing (NGS) is an advanced DNA sequencing technology that makes the simultaneous analysis of millions of DNA sequences possible for researchers. This process involves fragmenting the DNA and attaching adapters that are followed by amplifying and sequencing the DNA using different methods including Illumina, Ion Torrent, and PacBio. This process also allows the sequencing of entire genomes, transcriptomes, and epigenomes within minimum time and cost.

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