DNA sequencing determines the order of the four chemical building blocks- called "bases". These bases are responsible for making up the DNA molecule. This is something that helps scientists or researchers to figure out the specific kind of genetic information carried in a specific DNA segment. Many research support companies offer DNA sequencing services at affordable rates.
Let’s dive into Sanger Sequencing
Researchers often choose this specific technique to perform a low-throughput, targeted and short-read sequencing. Sanger sequencing is relatively simple and has sensitivity. This DNA sequencing technique, sanger sequencing, is the gold standard and this technology is used for a variety of applications ranging from targeting sequencing to confirming variants.
This is a well-known technique that utilizes a chain-termination method that helps to provide the order and identity of nucleotides in any strand of DNA. DNA sequencing utilizes the chemical analogs of four nucleotide bases. The analogs are called ddNTP. These are missing the hydroxyl group which is necessary for the extension of the polynucleotide chains that are responsible to form the DNA molecule.
Earlier, sanger sequencing was performed using capillary electrophoresis. This technology facilitates the separation of labeled DNA fragments by size, thin, acrylic-fiber capillaries, etc.
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