USA, 22nd March:
Microglia are central nervous system innate immune cells. Although numerous methods for isolating microglia from the brain have been developed, the method of dissociation and cell separationcan have a significant impact on the function of these highly dynamic cells.

We present an optimized Pluribead protocol for isolating CD11b+ cells (microglia) from mouse or human brain tissue. Isolated microglia can be used to model diseases with neuroinflammatory components in order to find potential therapeutics.

Tissue dissociation of murine brain
Tissue dissociation into single-cell suspensions with high cell viability and low cell debris is required for consistent cell separation, cell culture, and flow cytometry analysis.

PluriBead Technology
PluriBead separates cell mixtures using non-magnetic monodispersed microparticles (beads). Their surface is coated with monoclonal antibodies (mAb) that are directed at specific structures on the target cell's surface. During incubation, the target cells in suspension will bind to the pluriBeads and can then be separated from the suspension using a pluriStrainer (size exclusion).

Because the beads are larger than the cells, they cannot be phagocytized. There is no need for pretreatment of the blood (e.g. density centrifugation, erythrolysis, or other target concentration).

Key features of Pluribead

• No Sample Preparation: Use a sample volume of 200 l - 45 ml without gradient centrifugation, erythrolysis, or other procedures.

• Use any sample material: whole blood, buffy coat, PBMC, secretion/excretion material, brain homogenate, spleen, liver, and so on.

• Can be used on a Huge Range of Species: Isolate from humans, mice, rats, bovines, canines, sheep, and other species.

• Fast Isolation: Cell separation can be started in five minutes

• Gentle Isolation: A high yield of viable cells reduces the amount of sample required.

• Universal pluriBeads: can be used with any external antibody.

• Simultaneous Cell Isolation Using PluriBead Cascade: Separate two different cell types from the same sample material at the same time.

• Sequential Cell separation: Isolate up to six different targets from a single sample using sequential cell isolation.

PluriBead Principle

1. Mixing: pluriBead is added to the sample material.

2. Incubation: Target cells bind to pluriBead.

3. Separation: Unwanted cells are run through pluriStrainer - cell strainer Bound target cells to stay on top.

4. Detachment: Target cells are detached from the bead and run through a fresh sieve into a collecting tube.

With gentle and automated dissociation of brain tissue, viable single-cell suspensions free of myelin debris, and the ability to acquire pure and viable microglia from neonatal and adult mouse, rats, and humans, our products look to enhance your capabilities no matter your line of research.