Have you run into this problem before regarding any cell enrichment technique?

Consider the following scenario:You're researching a rare or restricted cell population. You prepare your sample and then perform fluorescence-activated cell sorting (FACS). It takes a long time. The longer it takes, the more cells die. You're having difficulty collecting enough cells for downstream applications. You're irritated. You wish there was an easier way. Perhaps a magic wand!

You’re not alone.

Sample preparation is critical to the success of downstream applications, but achieving high-quality results necessitates that samples be pure and free of contamination from harsh retrieval techniques. To keep up with technological advancements for assessing various aspects of cellular and molecular biology, there is a strong need for efficient, rapid, and gentle sample preparation methods.

Because current isolation techniques can be time-consuming and stressful to cells, developing a gentle and effective method to obtain a high purity of a small target cell population for downstream analysis is critical. That’s where Pluriselect comes in!

It’s not magic, it’s Pluriselect’s Unique Cell Separation Technologies!

Antibody supported cell separation

PluriSelect provides products for positive isolation via the pluriBead system, as well as negative isolation and depletion via the pluriSpin system.

Separation of Negative (Untouched) Cells
PluriSelect created the PluriSpin system, which isolates untouched and highly purified cells in a single step without the use of magnets or columns, reducing the risk of activating or damaging the cells of interest.

Positive Cell Separation
PluriBead cell isolation binds specific antibodies directly to the cells of interest. The targeting antibody will "touch" the cells. After isolation, the pluriBeads are separated.

Gravity supported separation

Our two different separation devices, pluriMate, and TwinSpin allow us to separate the target fraction regardless of whether the impurities have a higher or lower density.

pluriMate is designed to separate leukocytes and peripheral blood mononuclear cells (PBMC) from whole blood and bone marrow. The porous sponge incorporated at the bottom of the centrifuge tube is the key feature of pluriMate.

TwinSpin
TwinSpin centrifuge tubes prefilled with Density Gradient Medium (DGM) can be used to separate peripheral blood mononuclear cells from whole blood and bone marrow. The TwinSpin is made up of a standard 15 ml bottle and an inner tube.

Size Supported Separation
Pluriselect has the widest selection of straining/filtration devices. You can select the ideal device for your sample preparation in meh sizes ranging from 1 m to 1000 m, PET or nylon fibers, sterile or non-sterile, small volumes up to 800 L or in-line-strainer, and back-flush capability.

Let’s Connect!

We would love to learn more about your potential applications and discuss whether our unique cell separation technologies could be used to improve your workflow.