Cell isolation—also referred to as cell separation or cell sorting—is the process of isolating one cell population from other cells in a heterogeneous biological sample. Targeted cells are identified, isolated, and then separated by type.

What are some examples of cell isolation applications?

Cell isolation research is an essential component of many life sciences, ranging from stem cell and oncology research to routine clinical diagnosis.

Cell isolation techniques enable scientists to perform a wide range of tasks, including the following:

• Detect circulating tumor cells in the blood and perform molecular analysis on specific cell populations
• Preparing a blood sample separated from plasma to isolate immune cells from peripheral blood
• Separate bacteria from food and investigate the effects of drug candidates on different cell types.
• Separating the targeted population from the rest of the sample minimizes interference and is critical for identifying, studying, and analyzing specific cell populations.

Efficient Cell Separation Tools

It is always recommended that you select Cell Separation tools that will allow you to maximize efficiency while providing you with highly purified, functional cells for your research. Smarter, well-designed tools will allow you to get more out of your lab time.

Below mentioned are three of the cell isolation tools that we have developed with efficiency in mind:

1. Antibody-supported cell separation: Antibody-supported Cell separation is the process of directly enriching a specific cell population from whole blood, buffy coats, or any other biologic liquid, such as CD3 positive lymphocytes, CD14 positive monocytes, or CD19 positive B-cells. The antibodies can be linked to other antibodies, fluorescent dyes, or solid supports. PluriSelect offers the pluriSpin system for negative isolation and depletion and the pluriBead system for positive isolation.

2. Gravity Supported Separation: Density gradient centrifugation is the most common method for separating Peripheral Blood Mononuclear Cells (PBMC)-containing lymphocytes and monocytes) from red blood cells. PluriSelect offers optimized density gradient media for the isolation of Leukocytes, Monocytes, Platelets, and PBMCs from older blood, in addition to the most common solution for the isolation of PBMCs (density 1.077 g/ml).

3. Size Support Separation: Mesh-based separation according to cell size only works for cells with big size differences (like small and large fat cells). The Pluriselect Strainer family is perfect for sample preparation. Choose the ideal equipment for your sample preparation from meh sizes ranging from 1 um to 1000 um, sterile or non-sterile, PET or nylon fibers, back-flush capability, and small volumes up to 800 uL or in-line-strainer.

Choose the most efficient and effective method for your sample. Visit our website to learn more about our special offers and programs.