PluriMate was created to separate leukocytes and peripheral blood mononuclear cells (PBMC) from whole blood and bone marrow. It's a centrifuge tube that can be used for density gradient centrifugation. The porous sponge incorporated at the bottom of the centrifuge tube is the key feature of PluriMate.

This barrier is made of high-quality polyurethane. It saves you time and effort by eliminating the need to overlay the sample material. Anticoagulated blood or bone marrow can be poured directly into the pluriMate tube from the blood sampling tube. The porous barrier prevents the sample material from mixing with the separation medium.

Depending on the density gradient used (Leuko Spin Medium, Lympho Spin Medium, Lympho 24+ Spin Medium, or PLT Spin Medium), leucocytes, lymphocytes, and PBMCs are separated from unwanted erythrocytes and granulocytes during centrifugation and enriched in interphase above the separation medium. The barrier prevents recontamination of the enriched cell fraction during harvest after separation is complete.

Directions for the use of the PluriMate Tube

1. Ensure that all of the recommended medium, blood sample, density gradient medium, and centrifuge are at room temperature.

PluriMate Tube Preparation
2. Centrifuge for 10 seconds at 1000 x g, then discard supernatant (if there is any liquid above the barrier).

Include Sample Material
3. Place the sample material on top of the sponge.
PluriSpin PLT Depletion can be used to reduce platelet contamination.

Spin
4. Centrifuge at 800 x g for 15 minutes at room temperature with a swing bukket rotor and the brake on. If the blood is more than 4 hours old, centrifuge it for 30 minutes at 1000g.

Collect
5. Pipette until the white cell layer is removed.
6. Place cells from the white layer in a new tube.

Wash
7. Add wash buffer to the reaction tube.
8. Spin down cells for 10 minutes at 4°C with 300 x g (no or small brake).
9. Remove the supernatant and set the reaction tube on the table for 20 seconds. Excess wash buffer will run down the tube wall and collect at the bottom.
10. Most of the liquid above the pellet should be aspirated. The liquid will appear foggy because it contains mostly platelets; aspiration will improve the washing result.
11. Carefully pipette the pellet with 1 ml of wash buffer.
Steps 7–12 must be repeated.
13. Rehydrate the pellet to the desired volume.

You can find out more about PluriMate by visiting our website. By using our products, you can see the difference for yourself.